Issued August 31, 1916. 



U. S. DEPARTMENT OF AGRICULTURE, 



BUREAU OF ANIMAL INDUSTRY. 

 A. D. MELVIN, Chief of Bureau. 



SPECIAL REPORT 



F CATTLE. 



Conjunctival test (ophthalmic test) — O. T. 

 tul>ei-culin may be used but concentrated 

 precipitated tuberculin is preferred. Twen- 

 ty to 50 'r dilutions of tuberculin in pliysio- 

 logical salt solution are employed. The dried 

 puritied tuberculin (tablet form) is a con- 

 venieut form for use wlien conducting- this 

 test. 



Intracutaneous (intradermal) tt-st — ii.l c.c. 

 to 0.2 c.c. of a solution nf ( ». T. (ir jiurified 

 tuberculin may be used. 



GH, HICKMAN, LAW, 

 , PEARSON, 

 lOWER. 





Ke.spouMe to the Iii.U-ct ion <>t" 'I'liiiemiiiii. 



Siibc-utnueoiis Tiilieri-iiliu Uouotiuii — Char- 

 acterized by three features: 



(1) Constitutional reaction, consisting of 

 fever wliich is the most pronounced symp- 

 tom in cattle and accompanying symptoms 

 of lassitude, anorexia and rapid pulse. 



(2) A local reaction at the site of the in- 

 fection of varying intensity from sliglu red- 

 ness and tenderness to a severe inflammation 

 with adenitis. 



(3) A focal reaction about the tubercu- 

 lous lesion. 



These reactions do not run parallel and a 

 severe local reaction may occur with but 

 little or no constitutional disturbance and 

 particularly in the case of slight pulmonary 

 lesion.s symptoms of a focal reaction may not 

 be evidenced. 



Teclinio of Subcutaneous Tuberculin Test — 



For the subcutaneous test tuberculin may 

 be most conveniently administered subcutan- 

 eously in the prescribed dose by means of an 

 accurately graduated hvpodermic syringe, 

 just in front of or just back of the shoulder. 

 For the convenience and safety of the oper- 

 ator the elbows may be braced over the 

 withers of the animal and the injection made 

 on the side opposite to that on which the 

 operator is standing. 



N, 1916. 



\jyjV Cj IVIN .VI JC/ IN 



1916. 



NG OFFICE. 



