ANAEROBIC CULTURE 



89 



vaselined, inserted and twisted well home, and some 

 melted paraffin may be poured all round the joint and 

 melted in with a hot iron. The solutions in the bottle are 

 now well mixed, and the whole is placed in a suitable 

 incubator. The glass jars used for bottling fruit may be 

 employed instead of a stoppered bottle. The Buchner's 

 tube (Fig. 14) is convenient for single test- 

 tube cultures. It consists of a strong glass 

 test-tube, large enough to take an ordinary 

 test-tube, and having a constriction about 

 an inch and a half from the bottom. The 

 constriction supports the test-tube culture, 

 while the mixture of pyrogallic acid and 

 caustic potash fills the portion below the 

 constriction. A well-fitting rubber cork 

 closes the mouth of the tube, and the joint 

 may be paraffined for additional security. 

 If a Buchner's tube is not available, the 

 cotton- wool plug of the culture tube may 

 be pushed into the tube for an inch : some 

 solid pyrogallol is placed on the wool plug ; 

 this is just moistened with caustic potash 

 solution and the tube is stoppered with a 

 rubber cork or cap. 



Bulloch's apparatus is a bell-jar with FlG - l*. Buch 

 tubules, through which hydrogen may be 

 passed and a capsule containing alkaline 

 pyrogallol may be placed at the bottom. 



large 



enough 



n er ' s tube 

 arranged for 

 anaerobic cul- 

 tivation. 



to take Petri dishes or small 



It is 

 flasks. 



Mclntosh and Fildes l recommend the use of palladium 

 black. Asbestos wool, 0-25 grm., is soaked in 1-5 c.c. 

 of a 10 per cent, aqueous solution of palladium chloride, 

 rendered soluble by the addition of a little strong hydro- 



i lancet, April 8, 1916, 



