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A MANUAL OF BACTERIOLOGY 



chloric acid to the water. The wool is moulded with a 

 glass rod into a flat pad 1 in. square and dried. The 

 wool pad is then well smoked in a smoky gas flame and 

 heated in a blowpipe flame to reduce the palladium. 

 The prepared wool pad is enclosed in fine brass wire gauze 

 which is clipped in a bracket made of thin sheet brass so 

 that it can be suspended from the cork or lid of the 



FIG. 15. Mclntosh and Fildes' apparatus for anaerobic cultivation. 



apparatus. A glass bottle or a tin with lever top may be 

 used, and the cork or lid is provided with a tap through 

 which hydrogen is passed into the apparatus. Before 

 use the asbestos is heated in the Bunsen flame and 

 immediately placed in position in the tin or bottle, into 

 which the cultures have already been introduced. Hydro- 

 gen, which need not be purified, is then passed into the 

 apparatus, until increasing pressure stops the generation 

 of the gas or the tin is absolutely cold. The palladium 



