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A MANUAL OF BACTERIOLOGY 



and distilled water 1,000 c.c. The gonococci are decolorised, and 

 take up the red stain. In chronic urethritis the urine may be 

 centrifuged, and preparations are made from the deposit and 

 threads and stained ; it may be necessary to massage the pros- 

 tate in order to obtain secretion. 



In gonorrhceal vaginitis, etc., in the female, the discharge 



The Characters of the Chief Gram-negative Cocci (Gordon). 



acid. 



- = alkali. 



generally contains large numbers of other organisms, and the 

 gonococcus is usually difficult or impossible to detect. 



(2) Culture methods. Whenever a diagnosis is of great import- 

 ance an attempt should be made to cultivate the organism. Plate 

 cultures of agar smeared with blood as described (p. 299) and 

 another set with agar only should be prepared and incubated at 



