364 A MANUAL OF BACTERIOLOGY 



little or no effect is produced, but in a glandered animal, 

 about twelve hours after injection, the temperature rises 

 1-5 to 3 C. above the normal, a large and painful swelling 

 forms at the seat of inoculation (it may be as large as, 

 or even larger than, half a cocoanut). while any affected 

 lymphatic vessels or farcy buds become swollen. Reaction 

 may, however, be produced in the absence of glanders 

 if the horse is being treated with bacterial products, 

 toxins, etc. 1 



Epizootic lymphangitis has a superficial resemblance to 

 farcy in the horse, and must not be mistaken for the 

 latter (see " Sporotrichosis," Chapter XVI). 



The greatest care should be exercised when working with 

 glanders material or cultures, as several fatal laboratory 

 infections have occurred. 



Whitmore 2 describes a glanders-like disease occurring in man in 

 Kangoon ; especially among those addicted to the hypodermic 

 injection of morphine. A non-Gram-staining bacillus is present, 

 morphologically like the glanders bacillus, but killing guinea- 

 pigs with septicsemic symptoms and not affecting the testes, 

 growing well and luxuriantly on culture media, liquefying gelatin 

 slowly, growing well on potato with at first a cream-coloured, and 

 subsequently a yellowish growth, curdling milk and not ferment- 

 ing any sugar. 



Clinical Examination 



(1) Prepare film preparations of the pus or discharge, stain 

 with Lofifler's blue, and then partially decolorise in 4 per cent, 

 acetic acid. The ordinary pyogenic cocci will not be found 

 unless a secondary infection has occurred, and the material may 

 appear sterile, for the glanders bacilli may be very scanty. 



(2) Several tubes of glycerin-agar and potato should be inocu- 

 lated and incubated at 37 C. for seventy-two hours. On the 

 agar, colonies of the glanders bacillus will develop in twenty-four 



1 See Sudmersen and Glenny, Journ. of Hygiene, vol. viii. 1908, p. 14. 

 Journ. of Hycj., xiii, 1913, p. 1. 



