438 A MANUAL OF BACTERIOLOGY 



Other Continued Enteric -like Fevers 



Several members of the typhoid-colon group other 

 than the typhoid and paratyphoid bacilli occasionally 

 infect man with the production of diseases having some 

 resemblance to enteric fever. The principal of these are 

 B. alkaligenes, B. columbensis. B. asiaticus, B. archibaldi 

 and B. khartoumensis , the fermentation reactions of 

 which will be found in the table, pp. 450 et seq. These fevers 

 have been termed " parenteric " by Castellani and 

 Chalmers, e.g. " Alkaligenes Parenteric," etc. (see Manual 

 of Tropical Medicine, Ed. 3, 1919, p. 1408). B. (fcecalis) 

 alkaligenes is a typhoid-like, strictly aerobic bacillus. It 

 forms a brownish growth on potato and does not produce 

 indole. Milk is rendered alkaline, and the hexoses are 

 alone fermented with alkali production. 



Clinical Examination of the Faeces and Urine for 

 Typhoid and Paratyphoid Bacilli, etc. 



1. Preparation of the Fceces Emulsion, and Plating. 1 



The requisites are : test-tubes 3| in. x f in., bearing two file 

 marks indicating 9 c.c. and 10 c.c. ; platinum loops of stoutish 

 wire, the loops being 5 mm. internal diameter and bent at a slight 

 angle ; small watch-glasses or an artist's sunk porcelain palette ; 

 sterile tap-water or saline. 



Fill a test-tube with sterile water to the 9-c.c. mark. Add the 

 faeces with a glass rod so as to fill to the 10-c.c. mark. Emulsify, 

 mix and allow T to stand for one hour. 



Into each of four watch-glasses or cavities in the palette place 

 0-2 c.c. of sterile water or saline : label a, b, c, d. 



With the 5-mm. loop transfer a loopful from the upper layer of 

 the emulsion of faeces in the tube to a and mix. Transfer a 5-mm. 

 loopful from a to b, from b to c, and from c to d. These will now 

 be dilutions of faeces of approximately 1-100, 1-1,000, 1-10,000 

 and 1-100,000. 



1 Glen, Listen and Gore, 2nd. Journ. Med Research, 1919, Special Congress 

 No., p. 107. 



