ISOLATION FROM FAECES 441 



culture is Morgan's No. 1 Bacillus or a member of the Proteus 

 group. 



6. No change on mannitol and non-motile. Test with Shiga 

 serum. 



The emulsions are arranged in a test rack according as they 

 have been tested with P. GL mixture, P. T. mixture, Y serum, etc. 

 Equal volumes of serum (say from the stock P. G. mixture) are 

 placed in small " agglutination " tubes by aid of a large-bored 

 capillary pipette with rubber teat and fiducial mark, delivering 

 about 0-4 c.c. 



Similar volumes of the corresponding emulsions are added by 

 means of the same pipette, which is rinsed (between each opera- 

 tion) with boiling water from a beaker. As mentioned above, 

 the tubes, after being placed in the incubator, should be examined 

 every hour or so, and any that have reacted should be further 

 tested if necessary. No negative results should be recorded, 

 however, until the following morning, when the final readings are 

 made. 



Browning, Gilmour and Mackie's Method for Typhoid. Peptone 

 water (2 per cent. Witte's peptone and per cent, sodium chloride 

 in distilled water) is prepared, steamed for three-quarters of an 

 hour and filtered through paper. It is then distributed in test 

 tubes (6 in. by f in.), 5 c.c. in each, and autoclaved at 120 C. for 

 fifteen minutes. Six tubes of the peptone water are taken, and to 

 these are added respectively 0-04, 0-08, 0-12, 0-16, 0-22, 0-3 c.c. of 

 a 1-10,000 solution of brilliant green. A large loopful (up to 

 0-4 c.m. diameter) of faeces is then added to each tube and well 

 emulsified and mixed. The inoculated tubes are incubated at 

 37 C. for twenty to twenty-four hours, and then a loopful of the 

 contents is taken from each tube, and three successive streaks are 

 made on to a plate of MacConkey's medium. The plates are then 

 incubated and examined for the presence of the typhoid (or para- 

 typhoid) bacillus. The brilliant green (Bayer's brilliant green 

 extra cryst. the sulphuric acid salt, free from zinc) may be made 

 up as a 1 per cent, solution in distilled water and keeps for two to 

 three weeks. For use, the 1-10,000 solution is freshly prepared 

 by adding 0-1 c.c. of the stock solution to 9-9 c.c. of distilled 

 water. 



(See also Rajchman and Western's method, p. 456.) 



