ACTINOMYCOSES 



547 



periphery of the bodies ; these usually stain deeply with 

 the gentian violet, while the central portion of the body 

 is unstained and structureless, or contains granular 

 matter or calcareous particles. Various appearances may 

 be met with in different parts of the section, according as 

 the actinomycotic nodule is cut through its centre or 

 periphery ; when the latter is the case, the clubs are 



FIG. 50. Actinomyces. Film preparation of an 

 agar culture. Gram x 750. 



shown in transverse section and appear as closely packed, 

 deeply stained dots. Sometimes, however, in addition 

 to the clubs, the centre of the rosette is occupied by 

 numerous interlacing filaments, also stained by the 

 gentian violet. 



Nocardia bovis grows well aerobically at 22 C., but 

 better at 37 C. Anaerobic growths are, as a rule, but 

 poorly developed. 



352 



