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A MANUAL OF BACTERIOLOGY 



When cerebro- spinal fluid is to be tested, it is not inactivated, 

 and twice the amount (0-2 c.c.) is used for the test. 



[Harrison considers that inactivation at 56 C. for ten to fifteen 

 minutes suffices.] 



VI. PREPARATION OF THE KEAGENTS FOR USE. (a) When the 

 complement is standardised make up a sufficient quantity of the 

 correct dilution to allow 0-5 c.c. for each tube and a little over. 

 (6) Also make up diluted antigen as shown in the table, allowing 

 0-5 c.c. per tube for half the total number of tubes. 



VII. THE TEST PROPER. Mix the diluted antigen with an 

 equal quantity of diluted complement, and then, without delay, 

 measure 1 c.c. of the mixture into one of each pair of tubes 

 containing the sera. Shake. Incubate for one hour in the air 

 incubator (or for half an hour in the water-bath). 



Then add 0-5 c.c. of the corpuscle-amboceptor mixture to every 

 tube. Incubate again and read the results at the end of twenty 

 to thirty minutes. 



Mix the rest of the diluted complement with an equal quantity 

 of saline, and measure 1 c.c. of the mixture into the second tube 

 of each pair of tubes containing the sera. Incubate for one hour 

 in the air incubator (or for half an hour in the water-bath). 



If there be a large number of specimens, the mixture of antigen 

 and complement should be made in batches to avoid loss of 

 complement. 



[A serum is occasionally met with which gives partial or com- 



