ANTI-RABIC INOCULATION 



665 



and do not produce much constitutional disturbance. 

 At first there is a feeling of lassitude, and considerable 

 muscular tenderness at the seat of inoculation, which 

 later on passes off. At Lille, where only a few cases are 

 under treatment at a time, the cords, after drying for the 

 requisite period, are placed in pure sterile glycerin, in 

 which they retain their virulence unimpaired for about 

 a month, a rabbit being inoculated occasionally as 

 required. The system of dosage employed at the various 

 anti-rabic stations differs somewhat ; the following is 

 that employed at Lille, 2 mm. of cord being emulsified in 

 5 c.c. of sterile broth, or physiological salt solution : 



At Buda-Pesth a dilution method has been employed ; 

 instead of drying the cords, an emulsion is made with 

 the fresh cord, and this emulsion is considerably diluted 

 for the earlier doses, dilutions of 1 in 10,000 to 1 in 6,000, 

 corresponding to cords dried for from fourteen to eight 

 days. Semple l has found that a carbolised emulsion 

 of the cord may be employed as the inoculating agent. 

 An 8 per cent, emulsion of the cord in physiological salt 



1 Sc. Mem. Gov. of India, No. 44. 



