668 A MANUAL OF BACTERIOLOGY 



triturated and thoroughly emulsified in a sterile watch-glass by 

 means of a sterile glass rod with a bulbous end, a little sterile 

 broth being used to make the emulsion, and sufficient being 

 added to measure about 10 c.c. The hair on the head of a good- 

 sized rabbit is cut close, the animal is anaesthetised with ether, 

 the skin on the scalp reflected and a trephine hole made through 

 the skull. The centre of the trephine hole should be in the middle 

 line, and on the line drawn between the posterior corners of the 

 eyes, the diameter of the trephine being about ^ in. A little 

 of the emulsion is drawn up in a small syringe, having a fine 

 needle, and two or three drops are injected beneath the dura 

 mater. The operation is carried out with antiseptic precautions, 

 the wound closed, and a little wool and collodion dressing applied. 



If the material injected be from a rabid animal, the first symp- 

 toms will be noticed in from ten to fourteen days. The inocu- 

 lated animal loses control over its hind legs and throws them 

 about peculiarly when running. This increases, and in another 

 day or so the animal is apt to fall when running, and in another 

 day or two the hinder extremities become paralytic, and the 

 animal is unable to move, and dies shortly. The onset of 

 symptoms is hardly ever delayed beyond twenty-one days. 



Van Gehuchteris method. The ganglion is placed in absolute 

 alcohol for twelve hours, the alcohol being changed once ; it is 

 then embedded, and sections are cut. These are stained for 

 five minutes in Mssl's methylene-blue and mounted. Or the 

 material may be fixed in 10 per cent, formalin before staining. 

 The capsular changes are best shown by staining with hsema- 

 toxylin and eosin. 



Babes' method. A piece of the medulla or cord is hardened in 

 alcoho and stained with anilin red, and sections are prepared. 



Infantile Paralysis 1 



Infantile paralysis or acute anterior poliomyelitis occurs 

 sporadically and also in epidemics. 



Various organisms have been described in this disease, 

 but Levaditi, Landsteiner, and Flexner have proved 



1 See Levaditi, Journ. Roy. Inst. of Public Health, vol. xix, 1911, pp. 1 

 and 65 (Bibliog.) ; Flexner and others, Studies from the Rockefeller Institute, 

 1910 et seq. 



