LA\ KUAN'S SICKLES 171 



become free, and in this way he explains the occurrence of 

 tertian and quartan fever respectively, while quotidian fever 

 is dependent on the presence of both varieties, the new 

 spores of one kind being set free on the first day, and those 

 of the other on the second. 



In malarial cachexia there are found, besides the plas- 

 niodia, sickle-shaped bodies inside the blood-corpuscles 

 (' Litn-rttn'x strides '), and forms provided with flagella are 

 also occasionally observed (fig. 68). 



In the individual plasmodia an outer highly-refractive 

 part which easily takes the stain (ectoplasm) and an inner 

 part hard to colour (cntoplasm) are distinguished, the latter 



Vli:. 68. SKMII.fNAi: OK M< KU-:-SHAPKH MuDIKS, AMI I'KKK liupIKS I'KoVIDED 



WITH Fi.AiiKi.LA. (After Jaksch.) 



being enclosed by the former as by a ring. An excentrically- 

 placed nucleus containing nucleoli is found in the entoplasm 

 according to Mannaberg and other investigators. 



Artificial cultivation has not as yet been successful. It 

 has only been possible to preserve the plasmodia in the living 

 leech, in the digestive canal of which, as Eosenbach found, 

 they retain their vitality for at least forty-eight hours. 



The simplest mode of staining is that of Grassi and 

 Feletti, and is as follows : A small drop of malarial blood 

 is placed upon a cover-glass, which is inverted and laid 

 upon a slide carrying a drop of aqueous solution of methyl 

 blue or fuchsine; raising one side of the cover-glass a little 

 and letting it fall again suffices to effect mixture of the blood 



