APPENDIX 



BY THE TRANSLATOR 



A. l'<tccin<iti<>n (i<iinst Asiatic C1i<>l< r<i 



Principle of anti-cholera vaccination. The idea of vac- 

 cination against cholera is not a new one, as it is now some 

 years since Ferran professed to have discovered a cholera 

 vaccine. Other observers were, however, unable to satisfy 

 themselves of the genuineness of his results, and it is only 

 recently that a process established on a scientific basis 

 has been brought forward by M. Haffkine, of the Pasteur 

 Institute. 1 



During an attack of cholera the specific bacillus is only 

 found in the intestinal tract (see p. 144), and experiments 

 show that it dies when injected subcutaneously. The mor- 

 bid process must therefore be due to the absorption of 

 toxines generated by it, and Haffkine's vaccination aims at 

 acclimatising the system by the injection of an ' exalted 

 virus,' much stronger than any which it is likely to en- 

 counter in the ordinary way of infection, so as to enable it 

 to bear such quantities of cholera poison as may be ab- 

 sorbed from the intestine while an attack of the disease is 

 running its course. 



Preparation of the vaccine. The ' exalted virus ' is pre- 

 pared as follows : 2 A suspension, in about 3 c.cm. sterile 

 bouillon, of two or more standard cultures (twenty-four hours' 



1 The experiments (on different lines from Haffkine's) of Klemperei, of 

 Vincenzi, and of Brieger and Wassermann should, however, be also men- 

 tioned. 



- Wright and Bruce in Brit. Med. Journ. March 4, 1893, p. 227. 



