294 I3ACTERIOLOG V 



Griibler's powder is dissolved in 80 c.cm. of water, and 15 

 c.cm. of a 0*5 per cent, solution of acid fuchsine is added. 

 The sections are stained for at least an hour, washed in 

 water for half a minute, placed in 95 per cent, alcohol for 

 one minute and then for two to five minutes in absolute 

 alcohol, and finally transferred to xylol and xylol balsam. 



Sims Woodhead gives the following method of prepar- 

 ing and using this stain : 5 c.cm. saturated solution of 

 methyl green, 10 c.cm. saturated solution of methyl orange, 

 and 2 c.cm. saturated solution of acid fuchsine are mixed, 

 after having first been diluted with about 40 volumes of 

 water each, to avoid the formation of a precipitate. Stain 

 for fifteen minutes to twelve hours, rinse in 1 per 1,000 

 acetic acid, wash in dilute and then for one minute in abso- 

 lute alcohol, and finally immerse in xylol and benzol, and 

 mount in xylol balsam. 



The protoplasm of the epithelial cell is orange-red, the 

 nucleus green, the nucleoli brown or red ; the protoplasm 

 of the ' parasite ' is pale blue and the nucleus red. Con- 

 nective tissue is red, and leucocytes and all other cells are 

 orange-red with green nuclei. The protoplasm of the 

 'parasite' is, however, sometimes orange-red, and the 

 nucleus a little darker. Lastly, the living parasites may 

 sometimes be distinctly seen, according to Soudakewitch, 

 by examining scraped-off cancer cells in 0*6 per cent, salt 

 solution. 



(For bibliography, refer to the papers by Buffer and 

 Walker, and by Galloway.) 



Protozoa in other forms of new growth, Whether bodies 

 similar to those just described occur in sarcoma is still a 

 matter of doubt. Jackson Clarke, indeed, claims to have 

 found such, but the Morbid Growths Committee of the 

 London Pathological Society has decided } that his supposed 



1 See Report, Brit. Mcd. Journ., May 20, 1893, p. 1056. 



