BILE. 73 



10. From the alcoholic solution from which taurine 

 has been deposited, evaporate off the alcohol and dis- 

 solve residue in a little water. Remove hydrochloric 

 acid by a little silver oxyde, the latter by hydrothion, 

 and crystallise nitrate. Separate the crystals of glyko- 

 koll from the crystals of inorganic salts and examine 

 them. 



11. Add to crude or purified bile dissolved in water, 

 a little acetic or hydrochloric acid, and extract the 

 mixture with chloroform. Distil off the chloroform 

 and extract the residue with boiling alcohol. Fatty 

 acids and cholesterine will dissolve, and bilirubine will 

 remain undis solved, as a red powder. 



12. Add to crude bile a very small quantity of 

 acetic or hydrochloric acid, and shake with animal 

 charcoal until colourless. "Wash the charcoal with 

 water until the water comes away pure. Then 

 extract the charcoal with much boiling alcohol. Bili- 

 fuscine will go into solution and form a brown 

 liquid. 



13. Evaporate a weighed quantity of bile to dryness 

 and determine dry residue. It will be found to exceed 

 five per cent., and approach ten per cent, of the weight 

 of the bile. 



14. Burn a weighed quantity of dry bile with nitre 

 in a platinum dish, and in the residue determine sul- 

 phuric acid by baryta in the usual manner. From the 

 quantity of sulphur found calculate the quantity of 

 taurocholic acid present in the original bile. One part 

 of sulphur indicates 16*28 parts of taurocholic acid.. 



15. To some bile diluted with water and spread on a 



