BILE. . 75 



dyslysine, which contains, however, some undecom- 

 posed glykocholic and taurocholic acid. Decant and 

 filter the acid liquid, and evaporate to dryness repeat- 

 edly to expel all free acid. Then shake up residue in 

 spirit and let stand. Taurine will deposit in crystals ; 

 hydrochlorate of glykokoll and chloride of sodium will 

 remain in solution. Separate the latter by crystalli- 

 sation or by the process described under 10. 



19. Filter fresh bile through a cloth, or let stand for 

 twenty-four hours, and decant the clear portion. Place 

 in a stoppered bottle in a cool place or cellar for 

 several weeks or months. After that time filter red 

 liquid from deposit. The liquid contains a new acid, 

 which gives precipitates with the chlorides of calcium 

 and baryum, such as are not obtainable from fresh bile. 

 The deposit after washing and pressing should be 

 treated with boiling alcohol. Cholic acid will dissolve 

 and will deposit in crystals on cooling. Bilirubine, 

 and phosphates of lime, and magnesia with ammonia 

 in crystals, coloured greenish by impurity, will remain. 

 Extract the bilirubine by chloroform, and purify the 

 earthy salts by calcination, or analyse them by the 

 ordinary processes. 



Bilifuscme (probably C 9 H n N0 3 ). 1. Powder a 

 brown human gallstone and extract cholesterine with 

 boiling ether. Treat the powder with water and a 

 little hydrochloric acid, and wash it to neutrality. 

 Then extract again with boiling ether to remove fatty 

 acids, and boil the powder with absolute alcohol. 

 Bilifu seine will form a brown solution and remain after 



