76 BILIFUSCINE. 



evaporation of tlie alcohol as a black, shiny, brittle 

 mass, or as a dark brown powder. 



2. Dissolve a small quantity in dilute potash lye, and 

 reprecipitate by hydrochloric acid brown flakes. 



3. Dissolve in dilute ammonia, and add chloride of 

 calcium or baryum, when bilifuscate of calcium or 

 baryum will fall down in brown flakes. 



4. Observe that bilifuscine is not soluble in chloro- 

 form, and when exposed to the air in alkaline solution 

 does not yield biliverdine. 



5. Spread an alkaline solution on a white dish, and 

 add a drop of red nitric acid. Red, blue, green, 

 violet, and yellow coloured rings will be successively 

 produced. 



Bilirubine, synonym Cholophceine, C 9 H 9 N0 2 . 1. Ex- 

 tract some powdered oxgallstone successively with 

 water, alcohol, dilute hydrochloric acid, boiling alcohol, 

 and ether ; then boil the dry powder with dry chloro- 

 form, and exhaust with this agent. Distil the chloro- 

 form from the red solutions, but not quite to dryness. 

 To the residue add several volumes of absolute alcohol 

 and let stand twenty-four hours. There will be depo- 

 posited a brilliant red powder mixed with steel-blue or 

 brown crystals. Both the powder and the crystals are 

 pure bilirubine, and can be separated by levigation with 

 much absolute alcohol. After washing with alcohol 

 and ether, until the washings are purely yellow and 

 not green, the product is pure. Dry under the air- 

 pump. 



2. Human gallstones, by the foregoing treatment. 



