BLOOD. 83 



sulphuric acid and alcohol, and observe the formation 

 of a solution of hematine. (See Hematine.) 



13. Boil blood-corpuscles isolated by the salt-water 

 process under 3 with alcohol, and extract cholesterine 

 and lecithins. From the insoluble residue, decomposed 

 hematocrystalline, extract hematine by acid and alcohol, 

 and study albuminous residue like coagulated albu- 

 men. 



14. Evaporate the filtered ether solution obtained 

 under 4, and observe cholesterine and lecithine in the 

 residue. Identify cholesterine by its crystalline form, 

 and lecithine by its leaving on combustion free phos- 

 plioric acid. 



15. Treat blood-corpuscles isolated by the salt- 

 water process under 3 with water without stirring 

 much. The hematocrystalline will dissolve and a 

 gelatinous matter, the fibrino -plastic substance, will 

 remain undissolved. Add some ether and alcohol to 

 curdle the matter more completely, filter and wash, 

 The matter is soluble in solution of sodium chloride 

 and in water containing one tenth per cent* of hydro- 

 chloric acid. 



16. Collect a quantity of blood and, without stirring 

 it, let stand in a quiet cool place for twenty- four hours. 

 Remove the clear serum with a pipette or syphon, and 

 study as follows : 



17. Shake a portion with ether, and observe fats or 

 fatty acids in the residue of the ether solution. 



18* Mix a measured or weighed portion of serum with 

 several times its bulk of water, acidify with acetic acid, 

 and boil. The albumen of serum will be precipitated, 



