106 CHYLE. 



5. Separate the coagulum from chyle and observe 

 that it has all the properties offibrine. 



6. Treat the serum with ether; it will not become 

 clear. Add acetic acid or caustic potash to the serum, 

 and the ether will then extract all the fat. 



7. The addition of acetic acid in sufficient quantity 

 to the serum of chyle causes a precipitate of caseine. 



8. Boil the serum acidified with acetic acid and 

 filtered from the caseine, and observe that the albumen 

 is precipitated. 



9. In the filtrate prove the presence of peptones by 

 the reactions indicated under Chyme. 



10. Evaporate the filtrate to an extract, and pre- 

 cipitate all peptones by absolute alcohol. Evaporate 

 the alcohol from the filtrate. Remove fats by extrac- 

 tion with ether. Then extract by means of ether to 

 which some sulphuric acid has been added. The ether 

 leaves lactic acid on evaporation, to be purified and 

 tested as shown under that paragraph. 



11. Examine the extract for glucose by Trommer's 

 alkaline copper-solution. 



12. With the fats extracted under 10 some urea is 

 extracted. Separate this by water from the ether 

 residue and test with nitric and oxalic acid. 



13. Burn the extract of chyle and analyse the alka- 

 line ash. Observe its great similarity to the ash of the 

 serum of blood; it contains little iron and phos- 

 phoric acid, with lime and magnesia, but a considerable 

 amount of chlorine and alkalies, in the latter soda 

 prevails. 



