CHYME. 107 



Chyme. 1. Subject chyme to dialysis, and use the 

 dialysed liquid, after evaporation to a small bulk, for 

 the following tests, or 



2. Obtain peptones from fibrine, albumen, syntonine, 

 or gluten by artificial digestion with pepsine and dilute 

 hydrochloric acid, and use the resulting liquids for 

 these tests. 



3. Boil the solution ; it is not coagulated. 



4. Add pure taurocholic or glykocholic acid; in each 

 case a precipitate is immediately produced. 



5. Observe that the solutions turn the ray of 

 polarised light towards the left. 



6. Precipitate the solution with absolute alcohol, 

 the white flakes of precipitated peptone are again soluble 

 in very dilute alcohol. 



7. Add Trommer's copper solution to chyme or 

 peptone, and observe that it forms a purple fluid. 

 Boil, and if a red precipitate ensues glucose is present 

 in the chyme. 



8. Add cupric sulphate, ferric chloride, dilute 

 mineral acids to peptones, and see that no precipitates 

 ensue. 



9. Add chlorine, iodine, tannin, corrosive sublimate, 

 mercuric or mercurous nitrate, silver nitrate, neutral 

 or basic lead acetate, and observe that the peptones 

 give a precipitate with each of these reagents. 



10. Boil peptone with mercurous and mercuric 

 nitrite and nitrate with ultimately a slight excess of 

 nitric acid, and observe that a red precipitate and 

 solution results. 



11. Ascertain the composition of any peptone pro- 



