GLYKOGEN. 123 



calcium phosphate, and glycerine. It is more soluble 

 in cold than in hot water, and is precipitated by 

 alcohol. 



9. The lead salt is insoluble in water and alcohol. 



GlyJcocholic acid, C 36 H 45 N0 6 . 1. Add to some fresh 

 ox-bile a solution of neutral lead acetate, filter off the 

 precipitate, heat it with boiling alcohol, filter hot, pass 

 hydrothion into the filtrate while hot, and filter off the 

 sulphide of lead. Allow the filtrate to cool ; it will 

 solidify into a white crystalline mass of glykocholic acid, 

 which, after washing with water, is perfectly pure. 



2. Add to a small granule of glykocholic acid or of 

 its salts a drop of cane sugar solution, and then con- 

 centrated sulphuric acid in drops, agitating the solution, 

 and preventing overheating ; a violet or purple liquid 

 will be formed. 



3. To glykocholate of sodium dissolved in absolute 

 alcohol add ether ; a glutinous precipitate will ensue, 

 which on standing under the ether alcohol will trans- 

 form into crystals. 



4. To a glykocholate dissolved in water add hydro- 

 chloric acid ; a glutinous deposit of amorphous glyko- 

 cholic acid will ensue. 



5. Boil some glykocholic acid with excess of baryta 

 water ; cholate of baryum will crystallise after cooling, 

 and glylcokoll will remain in solution to be extracted as 

 stated under that substance. 



Glykogen, C 6 H 10 5 (Hepatine). 1. Mince a fresh 

 liver and boil with a small quantity of water. Strain 



