134 KREATININE. 



Kreatine, C 4 H 9 N 3 3 . 1. Take finely -chopped meat 

 free from fat, or chopped cod-flesh ; mix well with an 

 equal weight of water, squeeze in a bag of strong linen. 

 Boil the extract, filter off the coagulated albumen and 

 myochrome ; treat the filtrate with baryta water until 

 it has a strongly alkaline reaction ; filter, evaporate on 

 the water-bath to a syrup, allow to stand in* a warm 

 place. The liquid will spontaneously evaporate and 

 deposit crystals of kreatine, which can be purified by 

 boiling with animal charcoal and by recrystallisa- 

 tion. 



2. Observe the insolubility of kreatine in cold alcohol, 

 and its slight solubility in cold water. 



3. Boil some kreatine with a large excess of baryum 

 hydrate ; it is decomposed, and ammonia is evolved, 

 the vapour turning red litmus paper blue. This is due 

 to the formation and decomposition of urea on the one, 

 and the formation of alanine on the other hand ; both 

 substances can be obtained from the fluid. 



4. Dissolve some kreatine in some hydrochloric, 

 sulphuric, or nitric acid ; evaporate at a gentle heat ; 

 crystals are deposited which are soluble in alcohol, and 

 consist of a compound of the acid employed with 

 kreatinme. 



5. Dry crystallised kreatine in a current of hot air, 

 and observe that it loses water of crystallisation. 



Kreatinine, C^HfNgO. 1. Take fresh human urine, 

 not less than four litres, neutralise it with milk of lime 

 or hot saturated baryta water ; add a solution of 

 calcium chloride as long as a precipitate falls. Filter 



