KREATININE. 135 



and evaporate to a syrup, allow to stand, separate the 

 deposited crystals, and add to the syrup one twenty- 

 fourth of its weight of a neutral syrupy solution of 

 zinc chloride. Allow to stand three or four days ; the 

 double chloride of zinc and kreatinine is deposited in 

 crystalline, warty masses. Wash this deposit with 

 water. 



2. Dissolve it in boiling water ; add hydrated oxyde 

 of lead until the fluid has an alkaline reaction. Now 

 add three times as much oxyde of lead as has been 

 already used, and boil the whole for some time ; filter. 

 Boil the filtrate with some good animal charcoal, filter, 

 evaporate the filtrate to dryness ; a crystalline residue 

 will be left. Boil the crystals with eight to ten times 

 their weight of alcohol, allow to cool, decant the clear 

 liquid from any crystals of Jcreatine which may be de- 

 posited, and evaporate it, when crystals of Jcreatinine 

 will be deposited. 



3. Treat fresh urine with neutral lead acetate as long 

 as a precipitate is produced ; evaporate the filtrate to 

 one quarter of its bulk, and treat with hydrothion to 

 remove excess of lead. Expel hydrothion by boiling, 

 and add corrosive sublimate to the liquid. A white 

 precipitate of kreatinine mercuric chloride will fall. 

 Filter, wash, and decompose with hydrothion ; evapo- 

 rate filtrate to crystallisation, when kreatinine hydro- 

 chlorate will be obtained. 



4. Place a crystal of kreatinine on a slip of moist 

 turmeric paper ; it will produce a brown spot ; it has, 

 therefore, an alkaline reaction. 



5. Add to a solution of corrosive sublimate a solution 



