146 



LUTEINE. 



/. Add mercuric acetate ; a yellow precipitate 



will fall, leaving the liquid colourless. 

 g. Add copper acetate ; green precipitate. 

 h. Add lead or zinc acetate ; on standing all the 



yellow matter will go down as an oily layer. 



Examine with spectroscope with lime light. 



Two bands in blue and a third feeble one in 



violet will appear. 



^. 



AaBC 



Eb 



HH' 



Spectrum of ovario-luteine in alcohol. 



4. Set aside the alcohol solution to evaporate 

 spontaneously. The fatty residue will gradu- 

 ally deposit orange-red crystals of ovario-luteine. 

 Shake the mixture with absolute alcohol till the 

 fat is removed, then with repeated small quan- 

 tities of ether. Lastly, wash with ether, press 

 between paper, and dry. 



5. Reactions of ovario-luteine crystals. 



a. The crystals are soluble with a yellow or 

 orange colour in hot alcohol, ether chloro- 



AaBO 



Eb 



HH' 



Spectrum of ether solution of corpus luteum. 



