MUSCLES. 155 



9. Dissolve in dilute hydrochloric acid, and add 

 mercuric chloride ; only a slight turbidity will ensue. 



Muscles. Striated voluntary and involuntary muscles. 

 1. Examine under microscope. They will be found 

 to consist of fibres bound together in bundles, and 

 marked with transverse striae. 



2. Free the muscles of a recently-killed animal from 

 blood by injecting through the vessels a one per cent, 

 solution of sodium chloride. They must then be 

 frozen, minced, mixed with four vols. of snow con- 

 taining a little sodium chloride. The mass will liquefy, 

 and must be quickly filtered at 0C. The nearly clear 

 filtrate on regaining the ordinary temperature will 

 coagulate. Stir with a rod, and separate the coagulum 

 from the serum. 



3. Wash the coagulum with water, alcohol, and 

 ether. It will be found to consist of myosine (q. v.). 



4. Heat the serum to boiling : albumen will precipi- 

 tate in flakes. 



5. Extract the cake which remains after the extrac- 

 tion of myosine and albumen by process 2 with pure 

 water, and observe that the red colouring matter 

 myochrome, identical with hemato-crystalline, dissolves. 

 Study its properties as prescribed for hemato- 

 crystalline. 



6. Expose a piece of fresh muscle to oxygen under 

 a receiver, and observe that oxygen is absorbed and 

 carbonic acid evolved. 



7. After extraction of the myochrome (5), treat the 



