PEPSINE. 161 



3. Omicholine is insoluble in cold, slightly soluble in 

 boiling water, insoluble in alkalies when pure, very 

 soluble in alcohol or ether with a red colour. 



4. Heat a small portion on platinum foil ; a power- 

 ful urinous odour will be evolved. 



5. Examine the alcohol solution with the spectro- 

 scope ; a band in green will be observed. 



6. Examine with a lens in sunlight ; the solution 

 fluoresces green. 



Pancreatic Juice. 1. Notice appearance and alka- 

 line reaction. 



2. Add tannic or a mineral acid ; a white precipitate 

 will be produced. 



3. Heat to 72, a white precipitate will also appear. 



4. Add three or four volumes of 94% alcohol, filter, 

 dry the precipitate, dissolve in water, and filter. 

 Digest solution with some starch ; the latter will be 

 dissolved and converted into dextrine and sugar. 

 Digest with coagulated albumen ; it will dissolve. 

 With fats it will form an emulsion. 



5. Evaporate the juice to a low bulk and exhaust 

 with ether." The ethereal solution must be examined 

 for fats. The aqueous portion must be tested for 

 leucine and tyrosine (q. v.). 



6. Treat pancreatic juice with a little chlorine water ; 

 a reddish colour will be produced. 



7. Evaporate, burn, and analyse ash. 



Pepsine. 1. Treat the glandular layer of a stomach 

 with dilute tribasic phosphoric acid; filter and neutralise 



11 



