TJEINE. 179 



acid, dilute with a little water, filter the 

 solution through a small filter, and add to the 

 filtrate a little potassium sulphocyanide ; a 

 red colour confirms the presence of blood. 



2. Take 4 to 500 c.c. of the clear urine filtered from 

 coagulated albumen or sediment ; evaporate in 

 a porcelain dish or a water-bath to a thick 

 syrup ; divide the syrup into two parts, one 

 equal to one 3rd the other two 3rds of the whole. 



a. Extract the 3rd with strong alcohol ; filter, 

 and examine the filtrate. 



1. Evaporate a small portion nearly to dry- 

 ness and add a little nitric or oxalic acid, 

 and observe the crystalline forms of urea 

 nitrate or oxalate. 



2. Precipitate the larger portion with a few 

 drops of milk of lime and calcium chloride 

 solution and filter ; concentrate the filtrate 

 on the water-bath to 10-12 c.c. Transfer to 

 a beaker, add one-half c.c. of strong alcoholic 

 solution of zinc chloride, stir well and allow 

 to stand ; kreatinine chloride of zinc crys- 

 tallises out in warty grains. 



b. Acidify the two -thirds with hydrochloric 

 acid, and extract with ether. Evaporate the 

 ethereal solution and examine the residue for 

 hippuric acid. 



1. The filtrate will contain earthy phosphate 

 and other salts ; add ammonia ; the earthy 

 phosphates will be precipitated. 



