442 DISEASES OF CATTLE. 



killed rabbits and guinea pigs in as short a time as did the bovine 

 bacillus. Hogs which were inoculated subcutaneously with these 2 

 cultures from children died of generalized tuberculosis. Two calves 

 weighing over 300 pounds each were inoculated subcutaneously with 

 these virulent human cultures, and as a result developed generalized 

 tuberculosis. A yearling heifer inoculated with 1 of the cultures 

 showed generalized tuberculosis when killed three months after inocu- 

 lation. Both the cattle and the hogs had been tested with tuberculin 

 and found to be free from tuberculosis before the inoculations were 

 made. It is important to observe in this connection that 2 out of 4, 

 or 50 per cent, of the cultures obtained from cases of generalized 

 tuberculosis in children proved virulent for cattle. * 



Mohler, working in the Pathological Division, Bureau of Animal 

 Industry, has obtained 3 very virulent cultures of tubercle bacilli 

 from the human subject. A goat inoculated subcutaneously with 1 

 of these cultures died in 37 days with miliai-y tuberculosis of the 

 lungs involving the axillary and prescapular glands. This bacillus 

 was obtained from the mesenteric gland of a boy. Of still greater 

 interest is a bacillus isolated by Mohler from human sputum. A goat 

 inoculated subcutaneously with a culture of this germ died in 95 days 

 of pulmonary tuberculosis. A cat inoculated in the same manner 

 died in 23 days of generalized tuberculosis. A rabbit similarly 

 inoculated died in 59 days of pulmonary tuberculosis. Another rabbit 

 inoculated with a bovine culture for comparison lived 10 days longer 

 than the one inoculated with this sputum germ. Mohler also inocu- 

 lated subcutaneously a 1-year-old heifer with a culture derived from 

 the tuberculous mesenteric gland of a boy 4 years of age. This culture 

 was always refractory in its growth under artificial conditions, and 

 the bacilli were short, stubby rods^ corresponding in appearance with 

 the bovine type. At the autopsy, held 127 days after the inoculation, 

 the general condition was seen to be poor and unthrifty, and large, 

 hard tumors were found at the points of inoculation. On the right 

 side the swelling measured 3^ by 5 inches, and the corresponding 

 lymph gland was 2f inches long by If inches in diameter. This gland 

 contained numerous calcareous foci; one of these at the apex was an 

 inch in diameter. The lesions on the left shoulder of the animal were 

 very similar to those found on the right side, but the dimensions of 

 the tumor were slightly less. The lungs presented an irregular mass 

 of tuberculous nodules, and 7 or 8 grapelike nodules were seen on the 

 parietal pleura. Bronchial and mediastinal lymph glands contained 

 numerous tuberculous foci, and the pericardium, peritoneum, spleen, 

 and liver were also affected. 



In order to throw some light, if possible, upon the morphological 

 constancy of the different types of tubercle bacilli, Mohler has made 

 comparative studies of bacilli from various sources, and which had 



