the characteristic reactions for gallic acid. The poison, 

 if precipitated at all by lead acetate, must have gone down 

 in one of the preceding fractions. Later experiments showed 



that it is brought down partly mechanically and partly as a 



o 



lead compund in the first precipitates* 



FISETUr, 



Having identified gallic acid, and not finding any other 

 ph,en©l derivative in the lead precipitate, some of the ori- 

 ginal material was extracted with hot water to remove gallic 

 acid and filtered from tar while hot. The filtrate had a 

 deep yellow color. On cooling over night, an olive green 

 precipitate separated out which was dried and found to be a 

 light powder. It was practically insoluble in cold water, 

 soluble with great difficulty in boiling water from which 

 it separated in yellow flakes, slightly soluble in ether 

 and in acetic acid, but readily soluble in alcohol. The 

 solutions were not acid to litmus, gave a dark color with 

 ferric chloride, an orange-red precipitate with lead acetate 

 which was easily soluble in acetic acid, and an orange yellow 

 precipitate with stannous chloride. These properties and 

 reactions indicated that the substance was the dye-stuff 

 fisetin and that it occurs in the free state in this plant 

 though it is usually found as a glucoside of fisetin combined 

 with tannic acid. A compound of this kind was found in 

 -E8- 



