FECAL DIAGNOSIS OF INTESTINAL PROTOZOA 21 



L. tropica and L. americana can be obtained directly from 

 the sores. Films should be stained as described for the 

 malarial parasites. 



Special Literature on Leishmaniasis 

 LAVERAN, A.: Leishmanioses. 521 pp. Paris, 1917. 



3. INTESTINAL PROTOZOA OF MAN 



A. Methods -of Fecal Diagnosis of Intestinal Protozoa 



The method of fecal diagnosis employed depends some- 

 what on the accuracy of the results desired and the ability 

 to obtain and use special apparatus. The Donaldson iodin- 

 eosin method seems to be the quickest and easiest. Con- 

 centration methods give a slightly higher percentage of 

 positives and the Schaudinn iron-haemotoxylin smear 

 method is very useful in checking up doubtful cases. 



L Donaldson's iodin-eosin smear method. - a. Make up a 

 fresh solution as follows: Saturated solution of eosin in 

 normal salt solution, two parts; 5 per cent potassium iodid 

 in normal salt solution saturated with iodin, one part; nor- 

 mal salt solution, two parts. This is a modification of 

 Donaldson's stain, due to Kofoid, Kornhauser and Swezy. 



6. "The smear is prepared for microscopic examination by 

 rubbing out a minute bit of the feces by rolling it on a round 

 applicator stick in a small drop of normal salt solution and 

 then in an adjacent drop of iodin-eosin stain. A single cover 

 is placed on both drops and the smear is ready for immediate 

 examination. Living flagellates and unstained cysts appear 

 in the unstained part. In the stained area the bacteria, 

 fecal particles and the intestinal yeasts (except the larger 

 forms) stain at once. Against the pink background the 

 protozoan cysts stand out clearly as bright spherules which 

 soon become tinged with the iodin to varying tones of yellow, 



