INTESTINAL ENTAMOEBAE OF MAN 23 



95 per cent alcohol, 100 cc.; glacial acetic acid, 15 cc. Heat 

 to 65C. 



b. Make a smear on a slide and while still wet drop it 

 into the warm fixing solution. Leave there for about ten 

 minutes. 



c. Immerse in 70 per cent alcohol, containing a trace of 

 iodine 30 minutes to 24 hours; wash in water a few minutes; 

 immerse in 3.5 to 4 per cent aqueous solution of iron alum, 

 one to 4 hours; wash well in water; transfer to 0.5 per cent 

 aqueous solution of haematoxylin ; 4 to 24 hours. 



d. Differentiate in 1.75 to 2 per cent iron alum solution 

 until enough of the stain has been removed. This is best 

 done by examining under the microscope at frequent inter- 

 vals; wash well in a large amount of water; pass up through 

 alcohols to absolute; transfer to xylol; mount in balsam. 



e. Eosin may be used in the absolute alcohol if a counter 

 stain is desired. 



Special Literature on Methods of Diagnosis of Intestinal Protozoa 



BOECK, W. C.: A Rapid Method for the Detection of Protozoan 



Cysts in Mammalian Faeces. University of California 



Publ. in Zool., Vol. 18, pp. 145-149, 1917. 

 CROPPER, J. W., AND Row, R. W. H. : A Method of Concentrating 



Entamoeba Cysts in Stools. Lancet, Vol. 192, pp. 179-182, 



1917. 

 KOFOID, C. A., KORNHAUSER, S. I., AND SWEZY, O. : Criterions for 



Distinguishing the Endamoeba of Amebiasis from other 



Organisms. Archives of Internal Medicine, Vol. 24, pp. 



35-50, 1919. 



B. Intestinal Entamoebae of Man 



1. Classification. The Entamoebae belong to the class 

 Sarcodina. The members of this class are characterized 

 by the presence of locomotor organs in the form of tempo- 

 rary finger like projections of protoplasm called pseudopodia 



