Charles B. Lipman 



175 



flasks, diluted to proper volume, an excess of lye added, also 

 some powdered zinc to prevent bumping and the ammonia dis-' 

 tilling over was caught in ^ HC1. The excess of the latter 

 was titrated against ^ NH 4 OH; and the amount of nitrogen 

 fixed calculated in milligrams. Several sterile controls were run 

 with each series. The results of the first series follow: 



TABLE I. 



Every culture seems to show an increase of nitrogen except 

 nos. 10 and 13, but it may also be probable that the amounts 

 fixed in nos. 4, 5, 6 and 11 may lie within the limits of error, and 

 possibly even nos. 8 and 12 may be included in this list. Never- 

 theless, there is a distinct fixation of nitrogen in nos. 1, 2, 3, 7, 9 

 and 14, or in six out of fourteen cultures, and it is interesting 

 to note that of these six, three are true yeasts, one a mycoderma, 

 one a pseudo yeast and one a mold. The largest amount fixed 

 in this series was that in no. 9 by the " pseudo yeast" called 

 Tulare no. 46b. 



It would seem therefore that these results are a further confirma- 

 tion of those of Saida, Puriewitsch, Ternetz, Frohlich, Zikes and 

 others above mentioned, and it is especially interesting to note 

 that the organism in the series above given which showed the 

 largest fixation of nitrogen is very much like the Torula wiesneri 



