MUSCULAR TISSUE. 



The chief chemical constituents of muscular tissue may 

 be divided as follows: 



The proteins of which the two most important are myogen, 

 an albumin (not typical;, and myosin, a globulin. 



The nitrogenous extractives, comprising creatine, purine 

 bases, uric acid, carnine, and urea. 



The non-nitrogenous extractives, made up of glycogen, 

 dextrose, lactic acid, inosit, fats, and inorganic salts. 



Reaction. Test the reaction of living and dead muscle to 

 litmus and Congo-red paper. Explain the differences in the 

 results obtained. 



PROTEINS. 



MYOGEN. 



Place 25 grms. of hashed fresh muscle in a beaker with 75 

 c.c. of water and allow it to stand, with frequent stirring, for 

 one hour. Strain off the muscle through some cheese-cloth 

 (keep the residue), and filter the solution. 



Use this solution for the following tests: 



(a) What is the reaction of the solution? 



(6) Test the coagulability of the protein in the solution. 



(c) Perform three color protein tests. 



(d) Is the protein precipitated by MgSO 4 or (NH 4 ) 2 S0 4 ? 

 Why is myogen not a typical albumin or globulin? 



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