



3 



PREFACE. 



THE second edition of this Manual appears as the result 

 of a thorough revision of the original edition, which was issued 

 last year ; numerous additions to the subject-matter have also 

 been inserted. 



In its preparation the author has had iiv mind to provide 

 a guide for systematic work in a course in Physiological 

 Chemistry such as is given in the majority of the American. 

 Medical Schools. An attempt has been made to present, in 

 experimental form, all of the essentials of the subject. At 

 the same time, the experiments are simple and of a form 

 which would not overstep the possibilities of equipment and 

 time available in the average laboratory in physiological chem- 

 istry. Those facts which do not allow of simple experimen- 

 tal study have been ignored. The text preceding the experi- 

 ments has been limited to an explanation merely sufficient for 

 a correct understanding and performance of the work indi- 

 cated, with the expectation that the laboratory course will be 

 supplemented by lectures showing the important relationship 

 of the pure chemistry of the subject to physiological and 

 pathological processes. 



The description of the experiments has been set down with 

 considerable detail and, it is hoped, clearness, since the suc- 



iii 



578896 



