42 LABORATORY WORK IN PHYSIOLOGICAL CHEMISTRY. 



rhombic plates. The bile-acids also have a solvent action 

 on cholesterol. 



Make use of the cholesterol prepared in the course of the 

 separation of lecithin (p. 39). 



(a) Try the solubility of the substance in water. 



(6) Salkowski's Test. Dissolve a small quantity of the 

 cholesterol in a few c.c. of chloroform and add an equal 

 volume of concentrated H 2 S0 4 . The acid solution takes on 

 a greenish fluorescence and the chloroform becomes red. 



(c) Liebermann's Test. Dissolve a crystal of cholesterol 

 in about 2 c.c. of chloroform and add first 10 drops of acetic 

 anhydride and then concentrated H 2 S0 4 , drop by drop. The 

 mixture will become red, then blue, and finally green. 



