124 LABORATORY WORK IN PHYSIOLOGICAL CHEMISTRY. 



ties of albumin are demonstrable in urines with which the 

 more common tests yield negative results. 



(/) Spiegler's Test. Stratify 5 c.c. of urine which has 

 been slightly acidified with acetic acid, upon a few c.c. of 

 Spiegler's reagent. In the presence of albumin a white ring 

 appears at the line of contact of the two liquids. This 

 test is very sensitive, showing albumin in a dilution 1 : 250,000. 

 In fact most normal urines indicate protein with the reagent. 

 This must be borne in mind in making deductions from 

 its use. Urines containing iodides give a precipitate of HgL. 



(g) Tanret's (Bouchardai) Test. This reagent is added, 

 drop by drop, to 5-10 c.c. of the urine until a turbidity or 

 precipitate appears. The reagent precipitates besides al- 

 bumin, mucin, peptone, and alkaloids. In cases where the 

 presence of alkaloids in the urine is suspected, the peptone 

 and alkaloids may be dissolved in potassio-mercuric iodide 

 and the solution shaken out with ether, whereby the alkaloid 

 is dissolved. 



PROTEOSES. 



Non-coagulable bodies which are precipitated by satura- 

 tion with (NH 4 ) 2 S0 4 and which give the biuret reaction, 

 have been demonstrated and isolated from urines under 

 different conditions. These substances react positively to 

 nearly all of the proteose reactions, and it seems definitely 

 decided that true proteoses actually appear at certain times 

 in the urine. Of especial interest is the presence of an 

 albumose-like substance (Bence-Jones' body) associated with 

 multiple myelomata of the bone. 



The identification of these bodies follows from the use of 

 the same tests employed under Digestion, p. 54. 



