142 APPENDIX. 



MATERIAL. 



Fusion Mixture. 



5 parts Na^COa in substance and 1 part KN0 3 in sub- 

 stance, intimately mixed. 



Litmus Milk. 



Add a strong filtered solution of litmus to fresh milk 

 until the latter is decided bluish in color. 



Neutral Olive-oil. 



Ordinary olive-oil thoroughly shaken with a 10 per cent 

 solution of Na^COg; then extract the mixture with ether. 

 The residue after the evaporation of the ether is neutral fat. 



Oxalated Blood Plasma. 



As the blood flows from an artery, allow it to mix thor- 

 oughly in a beaker with an equal volume of 0.2 per cent 

 ammonium oxalate solution. 



Salted Blood Plasma. 



Allow the blood flowing from an artery to mix thoroughly 

 with an equal volume of a saturated solution of Na 2 S0 4 or 10 

 per cent NaCl. Place the mixture away in a cold place and 

 let it remain for 24 hours. 



Pancreatic Extracts. 



Proteolytically active: finely divided gland digested for 

 three days with water containing 5-10 c.c. of chloroform 

 to the liter, or with a saturated solution of NaCl. 



Amylolytically active: water or glycerol extract of the 

 gland previously allowed to stand exposed to the air for 



