CHAPTER II. 



PREPARATION OF CULTURE MATERIAL. 



ARTIFICIAL cultivations of micro-organisms in suitable 

 nourishing media in the incubator (Fig. 1) at temperatures 



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Tin. 1. INCUBATOR, WITH PAGE'S REGULATOR. 



A Page's Regulator. This consists of a tube filled with mercury, and im- 

 mersed in the water surrounding the chamber of the incubator. In the upper 

 part of the tube, above the mercurial column, is a fine open glass tube, having 



