si.] BACILLUS : PATHOGENIC FORMS. 93 



These bacilli are very minute, being of about the size of 

 tubercle-bacilli (see below), and are brought out by staining 

 the tissues with a concentrated watery solution of methylene- 

 blue, and after this washing with very dilute acetic acid. 

 They succeeded in artificially cultivating the bacilli in the 

 incubator at 38 C., on solid sterilised serum of horses' and 

 sheep's blood, using for the purpose particles (see below, under 

 " Tubercle-bacilli ") of nodules of the lung and spleen of a 

 horse dead of glanders. After two days (i.e. on the third day) 

 there appeared on the surface of the inoculated material the 

 first traces of the growth in the form of minute transparent 

 droplets which consisted entirely of the characteristic bacilli. 

 Cultivating these through several generations or transferences, 



FIG. 57. Pus OF A PULMONARY ABSCESS IN A HORSE DEAD OF GLANDERS. 



1. The nuclei of pus cells. 



2. The glanders-bacilli. 



Magnifying power 700. (The preparation had been stained with methylene-blue.) 



and then inoculating with them a horse, rabbits, guinea-pigs, 

 and mice, positive results were obtained, especially in the 

 guinea-pigs, which appear very susceptible to the disease. On 

 the site of the subcutaneous inoculation appears an ulcer with 

 indurated base, speedily enlarging and spreading ; other ulcers 

 follow in the neighbourhood, the neighbouring lymphatic 

 glands become swollen, and the general infection follows, in 

 the form of nodules and ulcers on the nasal septum, and 

 nodules in the internal organs. In the guinea-pig a charac- 

 teristic tumour of the testis, ovary, and vulva is often observed. 

 In all these cases the diseased tissues and organs contained the 

 characteristic bacilli 1 



1 Other writers on the bacilli of glanders are Drs. Bouchard, Capitan, Charvin, 

 in the Revu- medicate frnn^aise, Dec. 30. 1882 ; N. P. Wassilieff, in Deuitehe 

 vied. Woch. 11, 18S3, observed the bacilli in human glanders. 



