xi ] BACILLUS : PATHOGENIC FORMS. 95 



septicaemia known as fowl-cholera ; and the conclusion is 

 therefore forced upon us that Pasteur's cultures were contami- 

 nated with, or contained solely, the organism of this septicaemia. 

 Similarly his rabbits probably died from the same disease, 

 since these animals are exceedingly susceptible to septicaemia. 



On examining the diseased tissues of pigs dead of swine 

 plague by the modern methods of anilin staining, I ascertained 

 that all the diseased organs (lungs, intestines, inguinal and 

 bronchial lymph-glands) contain the characteristic bacilli, 

 mostly filling and plugging minute blood-vessels. So do the 

 diseased organs of mice and rabbits (spleen, liver, lung) dead 

 of the disease. 



Artificial cultivations made in broth and hydrocele fluid 

 from diseased organs of the pig, mouse, and rabbit, after an 



Fio. 59. FROM A PREPARATION OF BRONCHIAL Mucus OF A Pio DEAD or SWINS 

 PLAGUE. 



1. Detached epithelial cells of alveoli. 



2. Bacilli. 



3. Micrococci. 



Magnifying power 700. (Stained with Spiller's purple.) 



incubation of twenty -four hours at temperatures ranging 

 between 30" and 42 C. contain the above rods, which crowd 

 the nourishing fluids, all being rather short, about 002 to 

 0'003 mm. long, and all possessed of the power of active loco- 

 motion, such as is known to be possessed by the septic bac- 

 terium termo and bacillus subtilis. During the following days 

 of incubation, while the rods multiply, many of them lose 

 their motility, grow longer, up to 0'005 mm. and more, and 

 in some of the longer samples bright spores make their 

 appearance, one spore at one or both ends or sometimes in 

 the centre. 



