468 ^OIL SCIENCE 



Peptone starch agar, 5 days. 



Form : Irregular, round. 



Size: 1 to 1.5 mm. 



Enzymic zone : 2 to 3 mm. 



Elevation: Slightly convex. 



Consistency : Firm. 



Chromogenesis : White to light grayish white. 



Internal structure : Granular. 



Edge : Lacerate. 

 Beef agar, 5 days. 



Form : Round. 



Size: 1 mm. or less. 



Elevation: Slightly convex. 



Consistency : Butyrous ; old colonies become brittle. 



Chromogenesis : By reflected light colonies are gray white ; by 

 transmitted light they appear as light, semi-transparent smoky 

 drops. 



Internal structure : Finely granular. 



Edge : Entire. 

 Potato agar, 5 days. 



Form : Round. 



Size: 1 to 2 mm. 



Elevation : Convex. 



Consistency : Soft. 



Chromogenesis : Gray-white, sometimes showing concentric struc- 

 ture. 



Internal structure : Granular. 



Edge : Entire. 



11. Filter paper broths, 15 days. Paper reduced to a felt-like grayish white 



mass which breaks up into small particles on very slight agitation. 

 Paper destroyed more rapidly in solutions containing ammonium 

 sulphate, peptone, or potassium nitrate than in solutions containing 

 casein. 

 III. Biochemical Features. 



12. Dunham's solution, 10 days. No ammonia produced; nitrite produced. 



13. Starch nitrate broth, 10 days. No ammonia produced ; nitrite produced. 



14. Peptone nitrite solution, 10 days. Indol produced. 



15. Carbohydrate broths. No gas produced. Per cent of acidity (Fuller's 



Scale) with: Dextrose, 1.20; Lactose, 1.10; Saccharose, 1.00; 

 Maltose, 1.10; Glycerine, .00; Mannite, .00; Starch, .90. 



Pseudomonas mira, n. sp. 



Source: Soil from Corona, California; Glendora, California; Monrovia, California. 



I. Morphology. 



1. Vegetative cells: Average dimensions, 1.6 x .4 fx- 



2. Endospores : None observed. 



3. Flagella : 1 in number ; 4 to 6 yu. in length. 



4. Staining reactions : Gram negative. Stains readily with the atiiline 



dyes. 



II. Cultural Characteristics. 



5. Agar strokes, 5 days. 



Beef agar: Moderate, flat, grayish white, somewhat iridescent 



