APPENDIX VII. 325 



19. Pack eggs in moss at the first appearance of the eye- 

 spots, examine and note as before. 



20. Place eggs, as soon as taken, on ice, keep on ice, 

 and see how long they will be hatching. 



21. Freeze eggs solid, in water, at different stages of 

 development, and note the result. 



22. Freeze as before in the air. 



23. Place a few eggs on a copper-wire screen, and 

 note the discoloration and absorption of copper. 



24. Allow a few eggs, after the eye-spots appear, to re- 

 main considerably covered with sediment, and note the 

 deformity of the embryo when hatched. 



25. Subject eggs of different ages to high temperatures 

 of water, and note what degree of heat they will live 

 through. 



EXPERIMENTS WITH MICROSCOPE. 



With Milt. 

 2.6. Examine milt unmixed with water. 



27. Examine milt mixed with water. 



28. Examine changes of milt at 32 Fahrenheit 



29. Examine changes of milt at 36 Fahrenheit. 



30. Examine changes of milt at 40 Fahrenheit. 



31. Continue these experiments until a temperature of 

 80 is reached. 



32. Examine milt forced from fish by being placed in 

 warm water. 



33. Examine watery milt 



34. Examine creamy milt 



35. Examine action of blood on milt. 



With Eggs. 



36. Examine egg one day old. 



37. Examine eggs two days old. 



38. Continue experiments with eggs till hatching period 

 is reached. 



