18 THE LIVER, BY EWALD HERING. 



biliary passages of the liver in Rabbits, those also of Man do 

 not possess a membrana propria lined by hepatic cells, but are 

 immediately bounded by these cells themselves ; and the limit- 

 ing wall of the biliary duct, in the event of its isolation being 

 in any case accomplished, may be considered to be a condensed 

 surface layer of the cell substance, or a cell-membrane, or a cuti- 

 cula, which all amount to the same thing. 



The injection of the intralobular biliary passages is easily 

 accomplished in rabbits by throwing a solution of Prussian - 

 blue in water into the ductus choledochus, under a pressure of 

 20 30 millimeters of mercury (Mac Gillavry), especially if the 

 precaution be adopted of bleeding the animal to death, and 

 rendering the liver anaemic by opening the inferior vena cava. 

 In other animals I have not hitherto succeeded in injecting 

 the whole of a lobule. The method suggested by Chrzon- 

 szczewsky * is well adapted for the livers of animals 

 which are difficult to inject. It consists in the repeated in- 

 jection of indigo-carmine into the blood of the living animal; 

 after which the biliary ducts are found to be filled with the 

 colouring matter. 



The key to the right understanding of the course of the intralobular 

 biliary passages lies in the fact that they do not, as a general rule, run 

 along the borders, but between the planes of the hepatic cells ; and 

 inasmuch as my statements on this point have been already corrobo- 

 rated by Kolliker,! and in part also by Eberth,| I have made them 

 the basis of the foregoing account. The intralobular biliary passages 

 were first incompletely injected by Gerlach in the liver of the Pig, 

 and first completely injected by Budge || in the liver of the Sheep. The 

 latter, however, did not represent them as intercellular passages, 

 but as tubes provided with a nucleated membrana propria, in- 

 vested externally by the hepatic cells. MacGillavry coincided with 



* Yirchow's Archiv, 1866, Bandxxxv., p. 153. 



t Handbuch der Gewebelehre, 5th Edition, 1867. 



Loc. cit.j and in Schtiltze's Archiv fur Mikroskopische Anatomic, Band 

 iii., p. 432. 



Gewebelehre, 2nd Edition, 1854, p. 332. 



|| Reichert und Du Bois Raymond's Archiv fur Anatomie und Physio- 

 logie, Jahrgang, 1859, p. 642. 



