VACCINATION. IMMUNIZATION. INOCULATION 257 



and yellow and the pigeon dies in 12 to 48 hours; on post-mortem 

 examination a characteristic picture of cholera is found (hemor- 

 rhagic enteritis) and the specific cholera bacilli are recovered from 

 the blood. 



6. Swine Erysipelas. — Subcutaneous inoculation of mice 

 (skin pocket) or pigeons (breast) is followed in 24 hours by char- 

 acteristic sjrmptoms: dejection, roughened hair, dyspnoea, mucous 

 discharge from the eyes and agglutination of the eyelids. Mice 

 die in 2 to 4 days; pigeons in 3 to 4 days; the specific bacilli are 

 found in the blood. 



7. Strangles. — The most suitable inoculation animals are 

 white mice, which are immime to glanders. The inoculation is 

 made subcutaneously in the sacral region with a drop of pus or 

 nasal discharge. Death usually occurs in 3 to 6 days and on 

 post-mortem examination a pronounced picture of septicaemia is 

 presented (enlarged spleen, cloudy swelling of the internal organs, 

 exudate in the body cavities, blood infiltration at the point of 

 inoculation); in the blood, the specific strangles cocci are found. 

 More rarely, the animals die in 10 to 20 days with symptoms of 

 metatastic pyaemia. Field mice react only locally to strangles inoc- 

 ulation, which is contrary to their behavior to glanders inoculation. 



8. Rabies. — The best method is the intra-ocular inoculation 

 of rabbits with the brain substance of the suspected dog; the 

 material should be obtained in as fresh condition as possible. 

 After 12 to 14 days, the inoculated animal dies of dumb rabies. 

 Intramuscular and subconjunctival injection are also simple 

 methods, but the subdural (mtracranial, lumbar) and mtracerebral 

 inoculation of rabbits is more complicated. 



9. Hemorrhagic Septicemia. — Rabbits and mice die in 6 

 hours after cutaneous and subcutaneous inoculation, and in 12 

 to 24 hours after inoculation by feeding; large numbers of the 

 bacilli of hemorrhagic septicaemia are found in the blood; a further 

 characteristic in rabbits is hemorrhagic tracheitis. 



10. Malignant (Edema. — Only subcutaneous inoculations are 

 effective with mice and guinea-pigs; cutaneous inoculations are 



17 



