662 TECHNIQUE 



Mix the ingredients in the order given, rubbing the hematein 

 with the glycerin in a mortar. One or two drops of nitric acid 

 added to the final mixture will sharpen its properties as a nuclear 

 stain. 



This dye is used as a specific stain for mucinous tissues. It is 

 used in the same manner as hematein, and stains rapidly (three to 

 ten minutes). 



Mucicarmin (Mayer) : 



Carmin 1 grin. 



Aluminum chlorid 0.5 grm. 



Distilled water 2 cc. 



50 per cent, alcohol , 100 cc. 



Mix in the order given ; heat over a small flame till the fluid 

 darkens (two minutes) ; after twenty-four hours, filter. For use, 

 dilute with five to ten volumes of 50 per cent, alcohol. Like muc- 

 hematein, mucicarmin is a specific stain for mucus containing cells. 

 It also stains rapidly. 



Weigert-Pal Stain for Medullated Nerve Fibres. The tissues 

 must have been previously jfed in Mutter's fluid, washed in water, 

 hardened in alcohol, and sectioned. 



I. Stain : 



Hematoxylin 1 grm. 



Absolute alcohol 10 cc. 



Distilled water 90 cc. 



Saturated aqueous solution of lithium car- 

 bonate (1 to 80) 1 cc. 



II. Differentiating Solution : 



Potassium permanganate 0.25 grm. 



Distilled water 100 cc. 



III. Decolorizing Solution : 



1 per cent, aqueous solution of oxalic acid .... 50 cc. 

 1 per cent, aqueous solution of potassium sulfite 50 cc. 

 This last solution should be freshly prepared by mixing the two 

 stock solutions just prior to use. 



1. Stain sections (six to twenty-four hours) until black. 



2. Wash well in water. A few drops of lithium carbonate solu- 

 tion added to the water may improve the color, which should become 

 a deep blue-black. 



3. Differentiate until the grey matter becomes brown (one- 

 quarter to two minutes). 



4. Kinse in water. 



