SPECIAL STALLING METHODS 667 



1. Stain in Delafield's or Bohmer's hematoxylin, twenty to thirty 

 minutes. 



2. Wash well with water. 



3. Stain in Weigert's elastic tissue stain, twenty minutes. 



4. Wash in water. 



5. Stain in picro-fuchsin, three to five minutes. 



6. Rinse quickly in water. 



7. Dehydrate in absolute alcohol, clear, and mount. 



Mallory's Connective Tissue Stain. This method is applica- 

 ble only to tissues which have been fixed in Zenker's solution and 

 dehydrated with alcohol. Somewhat inferior results are obtained 

 after fixation with mercuric chlorid. 



I. Stain: 



Acid f uchsin 0.1 grin. 



Distilled water 100 cc. 



II. Fixative : + 



Phosphomolybdic acid 1 grm. 



Distilled water 100 cc. 



III. Counter- Stain: 



Anilin blue (soluble in water) 0.5 grm. 



Orange G (Griibler's) 2 grm. 



Oxalic acid 2 grm. 



Distilled water 100 cc. 



1. Stain in the fuchsin solution, three to twenty minutes. The 

 sections should become a bright red. 



2. Wash in water. 



3. Fix in the phosphomolybdic acid solution, one minute. 

 This prevents decolorization of the fuchsin. 



4. Wash well in water. 



5. Counter-stain in the blue solution, five to twenty minutes. 

 The sections should become decidedly blue. . 



6. Wash in water. 



7. Dehydrate, clear, and mount. 



Eosin and Methyl Blue Mixture (Mann) : 



1 per cent, aqueous solution of methyl blue. . 35 cc. 

 1 per cent, aqueous solution of bluish watery 



eosin 45 cc. 



Distilled water 100 cc. 



1. Mordant the sections in water, leaving them till all alcohol 

 has been replaced (five to thirty minutes). 



2. Stain in the above mixture, five to ten minutes. 



