468 NORMAL HISTOLOGY AND ORG ANOGRAPHY . 



2. For multipolar cells study sections of the cere-= 

 bral cortex and spinal cord stained with Cox-Golgi 

 method, as follows : 



Potassium bichromate 20 parts. 



Corrosive sublimate 5 per cent. 



sol 20 parts. 



Distilled water 40 parts. 



Potassium chromate, 5 percent. 



sol 16 parts. 



Specimens remain in this two weeks or two 

 months; after which wash thoroughly twenty-four 

 hours. Cut sections free-hand as thin as possible 

 and place them in a saturated solution of lithium 

 carbonate for twenty-four hours. Transfer to 95 

 per cent, alcohol, and then to clove oil, from which 

 they are mounted in balsam on a glass slide and 

 covered with a cover glass. 



3. Fix a sciatic nerve with 0.5 per cent, osmic acid. 

 Wash thoroughly in water and tease either in glyc- 

 erin or dehydrate and tease in oil. Study structure 

 of medullated fibers. 



4. Make cross section of sciatic nerve. 

 Blood. 



1 . Dip a thin strip of filter paper in the blood of a 

 frog and make a spread either on a cover glass or a 

 glass slide. Dry in air and fix in 95 per cent alcohol. 

 Stain with hematoxylin and eosin. Wash in water 

 and dry in air, after which the specimen may be 

 mounted in balsam. 



2. Study fresh specimens of human blood for 

 rouleaux and crenated red blood corpuscles. 



3. Thin blood spreads may be made : . 



(i) On glass slides by placing a drop of blood near 



