478 NORMAL HISTOLOGY AND ORGANOGRAPHY. 



in 3 to 5 per cent, aqueous solution of potassium 

 bichromate. Wash in water and transfer to the 

 following stain : 



Hematoxylin crystals 1 gm. 



Alcohol 95 per cent 10 c.c. 



Lithium carbonate, sat. aq. sol. 1 c.c. 

 Water 90 c.c. 



Dissolve the hematoxylin in alcohol first, and then 

 add the balance at the time of using. Stain the 

 sections in this for twenty-four hours. Wash in 

 water and transfer to a 0.25 per cent, fresh solution 

 of potassium permanganate for one-half to two min- 

 utes. Wash freely in water and place in the follow- 

 ing Pal solution : 



Oxalic acid 1 gm. 



Potassium sulphate 1 gm, 



Water 200 c.c. 



This will differentiate the gray and white matter 

 in one to three minutes. The nerve fibers should 

 stain blue. If the sections are too dark they may 

 be carried through the permanganate and Pal's so- 

 lution a second time. Transfer to water for several 

 hours, dehydrate, and mount in balsam. 



For a more complete description of laboratory 

 methods, including injections, fixing of tissues, and 

 special staining methods, see the following texts: 



"Pathological Technique/' Mallory and Wright, 

 Third Edition. 



" Microtomist's Vademecum," Lee. 



Bohm-Davidoff-Huber, "Histology," Second Edi- 

 tion, 1904. 



