PANCREATIC DIGESTION. 59 



latter consists of long white shining needles arranged in a 

 stellate manner, or somewhat felted (see " Urine," Fig. 32). 



(e.) Test for Tyro sin (Hofmann). Dissolve some crystals 

 by boiling them in water, add Millon's reagent, and boil, 

 which gives a rosy-red colour. 



(f.) Test a solution of ty rosin obtained by the prolonged 

 boiling of horn shavings and sulphuric acid, with Millon's 

 reagent, as in (e.) 



(g.) Indol. The remainder of the original digestive fluid 

 after digestion for ten hours or longer, emits an intensely 

 disagreeable odour, due to indol, whose presence is ascer- 

 tained by warming the liquid, and adding first a drop or two 

 of dilute sulphuric acid to some of the filtered liquid, and 

 then a very dilute nitrite solution. A red colour indicates the 

 presence of indol. This test is very readily obtained with 

 the products of digestion by Kiihne's dry pancreas (Lesson 

 VIII., 1, e). One must be careful to regulate the strength 

 of the acid. 



(h.) Acidify strongly with hydrochloric acid a small quan- 

 tity of the highly offensive fluid, and place in it a shaving 

 of wood or a wooden match with its head removed and 

 soaked in strong hydrochloric acid. The match is coloured 

 a beautiful red, sometimes even an intense red. The match 

 can be dried, and it keeps its colour for a long time, 

 although the colour darkens and becomes somewhat duskier 

 on drying. 



(i.) Chlorine Reaction. Add to some of the digestive 

 fluid (g, or preferably c), drop by drop, chlorine water, it 

 strikes a beautiful rosy-red tint. Or add very dilute bromine 

 water (1 to 2 drops to 60 cc. water), the fluid first becomes 

 pale red, then violet, and ultimately deep violet (Kuhne). 



III. The Action on Fats is twofold. 

 6. A. Emulsification. 



(a.) Rub up in a mortar which has been warmed in warm 

 water, a little olive oil or melted lard, and some pieces of 

 fresh pancreas. A creamy, persistent emulsion is formed. 

 Examine the emulsion under the microscope. Or use a 



