ABNORMAL CONSTITUENTS OF THE URINE. Ill 



(c.) If globulin be present along with serum-albumin, 

 make the urine alkaline with ammonia, allow it to stand for 

 an hour, filter, and to the filtrate add an equal volume of 

 a saturated solution of ammonia sulphate. A white floccu- 

 lent precipitate indicates globulin. 



5. Albumosuria. Albumose, which, however, is really a mix- 

 ture of four different proteids, has been found in cases of osteoma- 

 lacia. If such a urine can be procured, do test 2 (6.), using nitric 

 acid ; the deposit only takes place after a long time or on cooling, 

 and in fact the urine sometimes becomes almost solid, but is dis- 

 solved by heat. If there is a deposit, filter and test the filtrate 

 for proteid reactions e.g., the biuret test. It will give a preci- 

 pitate with acetic acid and potassic ferrocyanide. Then saturate 

 a portion of the urine with sodic chloride, and acidify with acetic 

 acid = a precipitate, which dissolves on adding much acetic acid 

 and heating, and disappears on cooling. 



6. Peptonuria. Peptone is frequently present in albuminous 

 urine. Peptone is most frequently present in urine in cases 

 where there is an accumulation and breaking up of leucocytes or 

 pus corpuscles, as in the stage of resolution of pneumonia, sup- 



furative processes, and in other diseases. Procure such a urine, 

 t is well to get rid of the albumin by acidification with acetic 

 acid and boiling. 



(a.) Put some urine in a test-tube, and by the contact 

 method pour on some Fehling's solution. At the line of 

 junction a phosphatic cloud is formed, and, if peptones be 

 present, above it a rose-pink colour. If albumins also be 

 present, a violet colour is obtained. Hemialbumose gives 

 the same reaction. 



(6.) Test the urine with tests 2 (b.) and (e.), and if they are 

 negative, and acetic acid alone gives a turbidity, suspect 

 peptone. Test a portion with acetic acid and phospho- 

 wolframic acid mixed with acetic acid. If peptone be present, 

 there is either immediately or after standing some time a 

 deposit. If there be no deposit on standing, peptone is 

 absent. It is better to precipitate the mucin with neutral 

 lead acetate, and then to apply the above test. 



7. Quantitative Estimation of Albumin. This can only be done 

 accurately by precipitating the albumin, drying and weighing it ; 



